| Antigen: |
CD163 (M130; Haemoglobin Scavenger Receptor) |
| Relevance: |
CD163 is a membrane glycoprotein of 130 kDa. It is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is a member of the scavenger receptor cysteine-rich superfamily, it is a receptor for the haemoglobin-haptoglobin complex |
| Clone: |
GHI/61 |
| Subclass: |
IgG1, kappa |
| Applications: |
Western blot, Immunohistochemistry (frozen sections), Immunoprecipitation, FACS |
| Immunogen: |
Hairy cell leukaemia cells |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Pulford, K Micklem K, McCarthy S, Cordell J, Jones M, Mason DY (1992) A monocyte/macrophage antigen recognised by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology 75:588-595 
Law SKA, Micklem KJ, Shaw JM, Zhang X-P, Dong Y, Willis AC, Mason DY (1993) A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol 23:2320-2325 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
For further information about antibodies from CRT or to discuss sales or licensing options, please contact us at reagents@cancertechnology.com
Click here to view Notes and Disclaimer information |
