| Antigen: |
CD3 |
| Relevance: |
The CD3 complex, composed of five CD3 polypeptide chains, associates with the T cell antigen receptor (TCR) and is found on all mature human T lymphocytes. CD3 can be used for the identification of normal and malignant T lymphocytes in paraffin embedded or frozen tissue sections. PC3/188A also detects CD3 in mammalian tissues. |
| Clone: |
PC3/188A |
| Subclass: |
IgG1 |
| Applications: |
Western Blotting, Immunohistochemistry (Frozen and parffin sections) |
| Immunogen: |
Peptide sequence (156-168) from the intracytoplasmic portion of the epsilon-chain of CD3 |
| Reactivity: |
Many animal species, including chicken, cow, guinea pig, horse, monkey, opossum, rabbit, rat and swine |
| Species raised in: |
Mouse |
| Fusion partner: |
P3/NS1/1-Ag4-1 (NS-1) |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
|
| References: |
Jones et al. 1993. J Immunol 150: 5429-5435  |
| Notes: |
This cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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