| Antigen: |
CD45RA |
| Relevance: |
CD45 is a transmembrane tyrosine phosphatase that is present on all leukocytes. CD45 regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45RA is an isoform of the CD45 complex which is selectively expressed on B cells |
| Clone: |
4KB5 |
| Subclass: |
IgG1, kappa |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections), Immunoprecipitation, FACS |
| Epitope: |
Cell surface |
| Immunogen: |
B cell lymphoma |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Pulford KAF, Falini B, Heryet A, Gatter KC, Mason DY (1987) 4KB5, A new monoclonal anti-B-cell antibody for the routine diagnosis of lymphoid tissue biopsies. In: McMichael A, et al (eds) Leucocyte Typing III, Oxford University Press, Oxford, New York and Tokyo, p 828 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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