| Antigen: |
Epithelial Membrane Antigen |
| Relevance: |
Marker for epithelial cells |
| Clone: |
E29 |
| Subclass: |
IgG2a |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Human milk fat globule membrane preparation |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, Mason DY (1985) Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Brit J Cancer 52:347-354 
Heyderman E, Strudley I, Powell G, Richardson TC, Cordell JL, Mason DY (1985) A new monoclonal antibody to epithelial membrane antigen (EMA) - E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Brit J Cancer 52:355-361 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
For further information about antibodies from CRT or to discuss sales or licensing options, please contact us at reagents@cancertechnology.com
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