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Anti-IgD Antibody Profile

Antigen: IgD
Relevance: For use as a primary or secondary antibody
Clone: IgD26
Subclass: IgG1
Applications: Immunohistochemistry (frozen and paraffin sections), FACS
Immunogen: IgD purified from human myeloma serum
Reactivity: Human
Species raised in: Mouse
Fusion partner: NS-1
Recommended growth conditions: RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:

Taylor CR, Mason DY (1974) The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin Exp Immunol 18:417-429 PubMed Logo

Naiem M, Gerdez J, Abdulaziz Z, Sunderland CA, Allington MJ, Stein H, Mason DY (l982) The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Meth 50:145-160 PubMed Logo
Notes: Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well

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