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Anti-TAL-1 Antibody Profile

Antigen: TAL-1 (SCL; TCL5)
Relevance: TAL-1 is a member of the helix-loop-helix family of transcription factors. Overexpression of the TAL-1 gene is the most common molecular abnormality found in human T cell leukaemia
Clone: 2TL242
Subclass: IgG1, kappa
Applications: Immunohistochemistry (frozen and paraffin sections), Immunoprecipitation
Immunogen: recombinant TAL-1 protein
Reactivity: Human
Species raised in: Mouse
Fusion partner: NS-1
Recommended growth conditions: RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:

Bernard M, Smit, L, Macintyre E, Matthieu-Mahul D, Pulford K (1995) Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain. Blood 85:3356-3357 PubMed Logo

Pulford K, Lecointe N, Leroy-Viard K, Jones M, Mathieu-Mahul D, Mason DY (1995) Expression of TAL-1 proteins in human tissues. Blood 85:675-684. PubMed Logo

Chetty R, Pulford K, Jones M, Mathieu-Mahul D, Close P, Hussein S, Pallesen G, Ralfkiaer E, Stein H, Gatter K, Mason D (1996) An immunohistochemical study of TAL-1 protein expression in leukaemias and lymphomas with a novel monoclonal antibody, 2TL 242. J Pathol 178:311-315 PubMed Logo

Chetty R, Dada MA, Boshoff CH, Comley MA, Biddolph SC, Schneider JW, Mason DY, Pulford KA, Gatter KC (1997) TAL-1 protein expression in vascular lesions. J Pathol 181:311-315 PubMed Logo

Notes: Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well

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