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Anti-CD11b Antibody Profile

Antigen: CD11b (Integrin aM; Mac-1 a subunit; C3bi receptor)
Relevance: Integrin aM is found in neutrophils, monocytes and NK cells.

CD11b plays a role in chemotaxis, apoptosis and phagocytosis.  It is absent in Leucocyte Adhesion Deficiency (LAD-1) and is a target for anti-inflammatory drug therapies

Clone: 2LPM19c
Subclass: IgG1, kappa
Applications: Immunohistochemistry (frozen sections) and FACS
Immunogen: purified C3bi receptor
Reactivity: Human
Species raised in: Mouse
Fusion partner: NS-1
Recommended growth conditions: RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:

Diamond MS, Garcia-Aguilar J, Bickford JK, Corbi AL, Springer TA (1993) The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J Cell Biol 120:1031-1043. PubMed Logo

Violette SM, Rusche JR, Purdy SR, Boyd JG, Cos J, Silver S (1995) Differences in the binding of blocking anti-CD11b monoclonal antibodies to the A-domain of CD11b. J Immunol 155:3092-101. PubMed Logo

Kupatt C, Habazettl H, Hanusch P, Wichels R, Hahnel D, Becker BF, Boekstegers P (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury. Arterioscler Thromb Vasc Biol 20:2226-32 PubMed Logo
Notes: Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well

For further information about antibodies from CRT or to discuss sales or licensing options, please contact us at reagents@cancertechnology.com

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