| Antigen: |
CD5 (Leu-1) |
| Relevance: |
CD5 is a surface glycoprotein present on peripheral T cells (>95%) and B-cell chronic lymphocytic leukaemia cells that binds CD72. CD5 has utility for the monitoring of T-cell numbers in peripheral blood and the identification of leukaemias of T cell origin. Absence of the CD5 marker on childhood ALL cells is associated with a more favourable prognosis. Detects CD5 on mammalian tissue |
| Clone: |
CD5/54/F6 |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Epitope |
Intracellular |
| Immunogen: |
Peptide from the intracellular region of CD5 (SSMQPDNSSDSDYDLHGAQRL) |
| Reactivity: |
Many animal species, including chicken, cow, guinea pig, horse, monkey, opossum, rabbit, rat and swine |
| Species raised in: |
Mouse |
| Fusion partner: |
P3/NS1/1-Ag4-1 (NS-1) |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Jones M, Cordell JL, Beyers AD, Tse AGD, Mason DY (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol 150:5429-5435.  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
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