| Antigen: |
HPV-16 E1/E4 (early proteins E1 and E4). |
| Relevance: |
The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer case. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer. |
| Clone: |
TVG 403 |
| Subclass: |
IgG1 |
| Applications: |
Immunohistochemistry (Frozen), ELISA, FACS |
| Immunogen: |
Antigen for hybridoma
production was expressed as a β-galactosidase
fusion protein using the pEX expression system and was
consequently cleaved to release the E1/E4 polypeptide. |
| Reactivity: |
Human papilloma virus |
| Species raised in: |
Mouse |
| Fusion partner: |
NSO |
| Recommended growth conditions: |
RPMI 1640 +10% FCS |
| References: |
Doorbar J et al. 1992.
Virology. 187:353-359 
Doorbar J et al. 2000. J. Virol. 74(21):10081-95 |
| Notes: |
The epitope recognised by this antibody survived brief fixation in 5% formaldehyde, but not prolonged fixation.10 kDa MW, the E1/E4 protein is aa 36-41 of human papilloma virus type 16. |
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