Description

Transgenic mouse models have been used to elucidate much information about vascular development. However, studies of protein function in angiogenic processes in later stages of embryonic development, as well as neo-angiogenesis in adulthood are restricted by the fact that gene knockouts are often embryonic lethal. As such, mouse models that allow both

temporal and spatial control of genes of interest are invaluable research tools for investigating protein function in these settings. Two mouse strains have been developed that express the inducible Cre-ER(T2) gene switch exclusively in endothelial cells. 

Background

Cre/LoxP is an established system for the silencing of specific genes in mouse models, utilizing the activity of the Cre recombinase to drive excision of the gene of interest, which is engineered with flanking LoxP target sites. In recent years, this system has been further improved through the use of fusions of Cre with the oestrogen receptor (ER), which are only active in the presence of tamoxifen, allowing temporal control of the tissue-specific recombination event. The improved ER(T2) version of the oestrogen receptor, developed by Metzger and Chambon shows increased sensitivity to tamoxifen, and lower response to endogenous steroids than the wildtype counterpart.

Download the PDF of Endothelial Tissue-specific and Inducible Cre Transgenic Mice

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