Description

This marker is a valuable tool for the rapid, non-destructive determination of pluripotency and early differentiation state of ES cell populations. 5T4 phenotyping will be useful for a wide range of techniques such as:

• Screening of culture reagents (including FCS, defined media, feeder cells, growth factors etc.) to establish optimal cell culture conditions for maintaining ES cell pluripotency or induction of particular lineage differentiation;
• Optimising transfer of pluripotent ES cell to improve chimera (germ line) production by ES cell knock out and knock in vehicles and for establishing and maintaining ES cell populations;
• Detecting compounds that induce stem cell differentiation

Activation of the 5T4 promoter could also be harnessed to induce expression of genes at the beginning of the stem cell differentiation pathway.

Download the PDF of Novel marker of early ES cell differentiation

For more information please contact

Dr Martyn Bottomley

+44 (0) 20 3469 6300 .(JavaScript must be enabled to view this email address)