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Relevance: In vitro study of branching morphogenesis of human mammary epithelial cells. Role of integrins in mammary morphogenesis.
Relevance: In vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.
Relevance: The stable human mammary MCF7-derived reporter cell line called AREc32, which contains a luciferase gene construct controlled by eight copies of the cis-element can be used to investigate whether anticancer drugs can induce ARE-driven gene expression.In these cells, luciferase activity is increased up to 50-fold following treatment with 50 µmol/L tert-butylhydroquinone (t-BHQ). Basal and inducible luciferase activities in AREc32 cells are increased by forced overexpression of Nrf2 and reduced by knockdown of endogenous Nrf2 expression with RNA interference. Depletion of cellular reduced glutathione (GSH) by treatment of AREc32 cells with L-buthionine-S,R-sulfoximine (BSO) does not influence basal levels of luciferase activity, but pretreatment with BSO augmented induction of luciferase activity by t-BHQ. Induction of reporter activity by t-BHQ in AREc32 cells can be suppressed markedly by the antioxidants N-acetylcysteine and GSH but only modestly by vitamins C or E. The anticancer drugs cisplatin, etoposide, mitoxantrone, chlorambucil, melphalan, and carmustine [1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU)] weakly induced luciferase activity in AREc32 cells. Moreover, treatment of AREc32 cells with BSO immediately before exposure to anticancer drugs enhances induction of ARE-driven luciferase activity by cisplatin, BCNU, chlorambucil, and melphalan and also induces endogenous AKR1C (AKR1C refers to AKR1C1 andAKR1C2), a target gene of Nrf2. Nrf2 can be activated by certain anticancer agents, and this will influence the effectiveness of chemotherapy.
Relevance: Embryonic Stem cell clones of C57BL/6 origin for use in generation of genetically modified mouse strains
Relevance: A MEF from the knockin of oncogenic Raf-1 D486A; in vivo study of oncogenic Raf-1 mutant and Ras signalling.
Relevance: In vivo study of Raf-1 knockout and Ras signalling. The cells can be used to carry out drug screening toxicology studies in MEF devoid of Raf-1.
Relevance: Highly metastatic subclone of murine epithelial tumour line CMT 64; in vivo mouse tumourigenesis and metastasis model; comparison of growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 64 line).
Relevance: In vivo mouse tumourigenesis system for study of growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 167 line); stable growth rate and morphology in culture and in lung metastasis.
Relevance: Packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation.
Relevance: Packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation.
Relevance: Primary human atypical mortal dysplasia oral cell line, which had an extended lifespan has been immortalised by ectopic expression of hTERT. Acquisition of immortality at the dysplasia stage or oral cancer progression is consistently associated with a number of changes; namely loss of retinoic acid receptor (RAR)-beta and p16 INK4a expression, p53 mutations and activation of telomerase.
Relevance: p300 is a transcriptional coactivator that functions as an integrator of numerous signaling pathways and are utilized by many DNA binding proteins to facilitate transcriptional activation. p300 shares numerous conserved domains with CREB binding protein (CBP), which is also a transcriptional coactivator. These shared domains include a histone acetyl transferase (HAT) domain, a bromo domain, and three cysteine- and histidine-rich domains. CBP also interacts with the RNA polymerase II holoenzyme and p300/CBP both contain transcriptional activation domains that function independently of HAT activity.
Relevance: This line can be used for the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments.
Relevance: HEK293 cells were transfected with GFP-WIPI1 then selcted with G418, and stable, amino acid responsive clones were selected.
Relevance: This cell line can be used to study autophagy by light microscopy. It is also useful for high throughput microscopy screens.
Relevance: Cell line derived from HEK293 stably expressing mRFP-tagged human Atg9 and rat GFP-LC3.
Relevance: In vitro study and comparison of genetic instability in myeloma tumour lines (homoplastic with JIM3); in vitro study of effects of DNA repair deficiency in myeloma tumour lines.
Relevance: In vitro study and comparison of genetic instability in myeloma tumour lines (homoplastic with JIM1); in vitro study of effects of DNA repair deficiency in myeloma tumour lines.
Relevance: The prospective commercial application of the line is in the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments.
Relevance: In vitro study of ERBB2 proto-oncogene over-expression in human mammary epithelia; in vitro study of the effects of ERBB2 over-expression on epithelial morphology & adhesion. The MTSV1-7 ce1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 ce1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and a2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
© Cancer Research Technology 2012