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Relevance: Cre under CYP1A1 promoter enabling inducible recombinase expression with administration of lipophilic xenobiotic (tissue-specific loxP knockout/knockin/transgene).
Relevance: Cre-ERT under Bmx promoter enabling inducible conditional recombinase expression in arterial endothelial cells (tissue-specific loxP knockout/knockin/transgene). The Bmx-Cre-ERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in arterial endothelial cells. The Bmx-Cre-ERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Relevance: Disease model for spontaneous epilepsy; in vivo studies of constitutively active form of MEK1, which is conditionally expressed in the murine brain and results in ERK activation. The caMEK1-CamKIICre transgenic mouse exhibits an epileptic phenotype, characterised by frequent, spontaneous seizures from 6-8 weeks of age. Seizures occur throughout the lifetime of the mouse, becoming less frequent in older animals. On average, caMEK1-CamKIICre mice have6.2 seizures per day. The caMEK1-CamKIICre mouse expresses a constitutively activated MAP/ERK Kinase (caMEK1) specifically in neuronal cells of the cortex, striatum and hippocampus of the mouse brain.
Relevance: Cdh5(PAC)-CreERT2 enable efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Relevance: In vivo study of Cyclin D1 kinase. The Cyl-1-/- mouse gives researchers the opportunity to study the contribution of Cyclin D1 to tumourigenesis and disease in combination with other genes through mating with tumour models, or other knockin / knockout / transgenic strains.
Relevance: Conditional knockout of Dll1; in vivo studies of tissue-specific Dll1 knockout and Notch signalling. The Dll1flox mouse is unique in allowing conditional knockout and analysis of the role of Delta like-1 in late embryonic, foetal or adult tissues, and is an ideal tool in the study of Delta like-1 and Notch signalling. The Dll1flox mouse enables tissue-specific knockout of Delta like-1, a principle ligand of Notch controlling cell cell fate in multiple tissue types (neural, inner ear, haematopoietic, lymphoid, epidermal, intestinal epithelia and pancreas). Dll1flox carries loxP-flanked exons within the Dll1 locus, allowing knockout of the Dll1 gene when mated to an appropriate Cre deleter mouse.
Relevance: A KO B6(Cg)-Clec9atm1.1Crs mouse strain. These mice may be useful for studying adaptive immune responses to antigens in apoptotic and necrotic cells and for tracking DNGR-1+ dendritic cells. The KO mice have a deficiency in the ability to cross present dead-cell associated antigens.
Relevance: In vivo study of DR3-induced apoptosis & DR3 function; in vivo study of negative T cell selection & development.
Relevance: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
Relevance: Endoglin is an auxiliary receptor for TGFb signalling. Heterozygous germline Endoglin mutations have been identified in patients with the vascular abnormality, Hereditary Haemorrhagic Telangiectasia. Endoglin is upregulated in endothelial cells during angiogenesis and the loss of Endoglin in [transgenic] mice results in embryonic lethality at mid-gestation. This phenotype points to an important role of Endoglin in new blood vessel formation but precludes analysis at later stages in development and in postnatal life. To bypass this limitation and allow further investigations of the function of Endoglin a transgenic mice has been generated with a floxed Endoglin allele in which loxP sites flank exons 5 and 6. Mice homozygous for this allele are normal and in the presence of appropriate Cre lines will allow time and cell specific Endoglin deletion for in vivo analysis of function in cardiovascular development and disease.
Relevance: Germline mutations in the fumarate hydratase tumour suppressor gene predispose to leiomyomatosis, renal cysts, and renal cell cancer (HLRCC). Fh1-deficient mice develop renal cysts that have an activated hypoxia pathway, with overexpression of Hif1alpha and Hif2alpha. Hif targets, such as Glut1 and Vegf were also upregulated. Therefore the mouse may be useful for testing therapeutic interventions that target angiogenesis and HIF-prolyl hydroxylation.
Relevance: In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)
Relevance: The APC mouse is an established and commercially available model of colon cancer. Crossing the APC mouse with the GSTPnull mouse enhances the phenotype making in more reproducible in terms of number of papilloma and subsequent tumour development, earlier development of papilloma and tumours. In addition, the tumours have higher tendancy to be located in the colorectal region - rendering the model more clinically relevant.
Relevance: Disease model for psoriasis; in vivo study of human a2 and b1 integrin transgene expression in skin.
Relevance: Disease model for psoriasis; in vivo study of human a3 and b1 integrin transgene expression in skin.
Relevance: Disease model for psoriasis; in vivo study of human a5 and b1 integrin transgene expression in skin.
Relevance: Chemically-induced skin tumourigenesis model; in vivo study of a6 and b4 integrin transgene expression in skin.
Relevance: Disease model for systemic lupus erythematosus; in vivo study of IFNg expression in skin.
Relevance: Disease model for psoriasis; in vivo study of constituitively active murine MEK1 mutant (S217E/S221E) in skin.
Relevance: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
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