Anti APC Clone Ali 12-28 Profile

Antigen: Adenomatous Polyposis Coli (APC)
Clone: Ali 12-28
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: N-terminal APC-MBP fusion protein
Epitope:

Region corresponding to nucleotides 135 and 422 of the APC (exons 2-3) in the N-terminus.

Positive Control:

Cancer cell lines: SW480, LOVO, DLD1, SW837

Cross Reactivity:

Human

Applications: Acetone, ELISA, Frozen, Immunoprecipitation, Western
Growth Conditions:

DMEM + 5% FCS

Relevance: The adenomatous polyposis coli (APC) tumour suppressor gene is mutated (often by deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and in over 80% of colorectal cancers. Ali 12-28 may be used for the detection of APC expression and APC mutations.
References:

Efstathiou JA et al. 1998. Proc Natl Acad Sci USA. 95(6):3122-7. PMID: 9501226 Read more

Notes:

Ali 12-28 detects both normal and mutant-truncated forms of the APC. Recommended concentrations for western blot: 4 �g/ml. Immunofluorescence:  20 �g/ml