| Antigen: | Human APC15 |
| Clone: | APC15 |
| Species Raised In: | Guinea Pig |
| Immunogen: | Full length His-TEVhAPC15 purified from BL21 E.coli |
| Positive Control: | Asynchronous human cell lysates |
| Cross Reactivity: | Human |
| Applications: | Immunofluorescence, Immunoprecipitation, Western |
| Synonyms: | In Schizosaccharomyces cerevisiae APC15 is called Mnd2 (PM gene ID: 854843) |
| Relevance: | The uncharacterised open reading frame C11orf51 has been identifi ed in a systematic proteomic analysis of APC/C purifi ed from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to S. pombe APC15, and S. cerevisiae Mnd2. hAPC15 is previously uncharacterised. It has been shown for the fi rst time that human APC15 is a component of the Anaphase promoting complex/cyclosome (APC/C) which is required for progression from metaphase during cell cycle. Specifi cally, APC15 drives the turnover of mitotic checkpoint complexes (MCC)-Cdc20 to make the spindle-assembly checkpoint responsive to kinetochore attachment. Depleting APC15 prevents Cyclin B1 ubiquitylation and degradation because MCCs are locked onto the APC/C and cannot be released when all the kinetochores have attached to the spindle. |
| References: | Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. Read more |
© Cancer Research Technology 2012