Anti ASAP1 Clone 7B12 Profile

Antigen: ASAP1
Clone: 7B12
Subclass IgG2b
Species Raised In:

Mouse

Immunogen: Recombinant Protein (fragment of human ASAP1 (corresponding nucleotides 977-1532 of KIAA1249) with N-terminal GST tag produced in E.coli strain BL-21)
Positive Control:

Human: HT29 and MDA-MB-231
Rat: ASAP1 is strongly expressed in ASML and weakly expressed in 1AS pancreatic carcinoma cells

Cross Reactivity:

Human, Rat (Not tested in Mouse)

Applications: ELISA, Frozen, Immunohistochemistry, Paraffin embedded, Western
Growth Conditions:

10% FCS + RPMI + Pen/Strep

Fusion Partner: Sp2
Synonyms: Centarin beta 4, Def1, DDEF1, PAG2, AMAP1
Relevance: ASAP1 possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. It potentially has involvement in tumour progression, having been shown to promote metastasis formation in vivo and stimulate tumor cell motility, invasiveness, and adhesiveness in vitro. ASAP1 represents a potential target for cancer therapy.
References:

Mueller et al. 2010. Oncogene. 29:2393-403. Read more

Notes:

Other research areas applicable: Metastasis, cytoskeleton, vesicle trafficking.
Suggested Dilutions: WB: 1ug/ml, Immunostaining: 5ug/ml, IP: 10ug/ml