Anti Alkaline phosphatase Clone AAP1 Profile

Antigen: Alkaline phosphatase
Clone: AAP1
Subclass IgG2a
Species Raised In:

Balb/c

Immunogen: D98/AH-2 (HeLa) cells, which produce human intestinal alkaline phosphatase ectopically
Cross Reactivity:

Human

Applications: ELISA
Growth Conditions:

DMEM + 5% FCS

Fusion Partner: P3X63Ag8
Synonyms: ALP1, IAP (ntestinal alkaline phosphatase), Kasahara isozyme antibody
Relevance: Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
References:

Arklie J et al. 1981. Tissue-Antigens. 17(3):303-12. PMID: 7314066 Read more
Brock DJ et al. 1985. Lancet. 1(8439):1175-8. PMID: 2860384 Read more

Notes:

AAP.1 may be used in the diagnosis of tumours as some tumours produce intestinal ALP ectopically.