| Antigen: | Alkaline phosphatase |
| Clone: | AP-7/6/7 |
| Subclass | IgG1 |
| Species Raised In: | Mouse |
| Immunogen: | Calf intestinal alkaline phosphatase |
| Cross Reactivity: | Calf |
| Applications: | |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | NS1 |
| Relevance: | Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. |
| References: | Cordell JL et al. 1984. J Histochem Cytochem 32:219-29. PMID: 6198355 Read more |
| Notes: | AP-7/6/7 is recommended for APAAP immuno-alkaline phosphatase technique. Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
© Cancer Research Technology 2012