| Antigen: | CD79a |
| Clone: | HM47 |
| Subclass | IgG1 |
| Species Raised In: | Mouse |
| Immunogen: | Synthetic peptide of 14 amino acids representing amino acids 202-216 of the human mb-1 cDNA sequence. |
| Cross Reactivity: | Human, monkey, pig, horse, rabbit, cow, guinea pig, rat, mouse, opossum and chicken. |
| Applications: | |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well. |
| Fusion Partner: | P3-NS1/1-Ag4-1 |
| Relevance: | The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections. |
| References: | Mason et al. 1991. J Immunol. 147:2474-82 PMID: 1747162Read more |
© Cancer Research Technology 2012