| Antigen: | Anti-Silencing Function 1 (ASF1) |
| Clone: | ASF 4A1/3 |
| Subclass | IgG2a |
| Species Raised In: | Balb/c |
| Immunogen: | FLAG-ASF1A |
| Cross Reactivity: | Human, Mouse, E. coli |
| Applications: | Western |
| Growth Conditions: | DMEM + 5% FCS |
| Fusion Partner: | Sp2/0Ag.14 |
| Relevance: | Anti-silencing function 1 (Asf1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled-like kinase (TLK). The Tousled kinases are themselves regulated by the checkpoint protein kinases involved in the DNA damage response. |
| Notes: | ASF 4A1/3 detects both ASF1A and ASF1B. |
© Cancer Research Technology 2012