Anti Band III Clone Q1/156 Profile

Antigen: Band III
Clone: Q1/156
Subclass IgG1
Species Raised In:

Mouse

Immunogen: Foetal liver cells
Cross Reactivity:

Human

Applications: Frozen
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Synonyms: Band 3 anion transport protein, Anion exchange protein 1, AE1, Solute carrier family 4 member 1, SLC4A1, DI, EPB3
Relevance: Band III is a protein associated with the surface membrane of red blood cells and their precursors. It is the major glycoprotein of the erythrocyte membrane and mediates exchange of chloride and bicarbonate across the phospholipid bilayer and plays a central role in respiration of carbon dioxide. Band III is also a potential binding site for haemoglobin.
Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.