| Antigen: | CD2 |
| Clone: | X53 (X/3) |
| Subclass | IgG1 kappa |
| Species Raised In: | Mouse |
| Immunogen: | T cell lymphoma cells |
| Cross Reactivity: | Human |
| Applications: | FACS, Frozen |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | P3/ NS1/1-Ag4-1 |
| Synonyms: | T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, SRBC |
| Relevance: | T-cell surface antigen CD2 is an antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. CD2 interacts with lymphocyte function associated antigen (LFA3/ CD58) and CD48/BCM1 to mediate adhesion between T cells and other cell types. Intracellularly, it interacts with lck. CD2 can be used for monitoring T-cell number in periperal blood, and identification of lymphomas and leukaemias of T-cell origin. |
| References: | Pawelec G et al. 1989. Stimulatory capacity of Workship T-cell antibodies on T-lymphocyte clones. Leukocyte Typing IV, Oxford Univeristy Press, Oxford, New York and Tokyo; p310-311.2.CD guide for CD2 (1989) Leukocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo; p1074-1075 |
| Notes: | This cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
© Cancer Research Technology 2012