Anti CD20 Clone PDR78 Profile

Antigen: CD20
Clone: PDR78
Subclass IgG
Species Raised In:

Mouse

Immunogen: Pokeweed mitogen-activated Burkitt's lymphoma cell lines.
Cross Reactivity:

Human

Applications: FACS, Frozen
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Synonyms: MS4A1
Relevance: CD20 is a non-Ig differentiation antigen of B cells. Oligomer of CD20 act as calcium channels. It may also be involved in B cell activation and cell cycle progression. CD20 is broadly expressed by normal and neoplastic B cells and absent from all other leucocytes and tissues.
References:

Ono N et al. 1997. CD20 Workshop Panel report. In Kishimoto T et al (eds) Leucocyte Typing VI. Garland Publishing Inc., New York and London, p 135-137.

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.