| Antigen: | CD3 |
| Clone: | PC3/188A |
| Subclass | IgG1 |
| Species Raised In: | Mouse |
| Immunogen: | Peptide sequence (156-168) from the intracytoplasmic portion of the epsilon-chain of CD3 |
| Cross Reactivity: | Many animal species, including chicken, cow, guinea pig, horse, monkey, opossum, rabbit, |
| Applications: | Frozen, Paraffin embedded, Western |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | P3/ NS1/1-Ag4-1 |
| Relevance: | The CD3 complex, composed of five CD3 polypeptide chains, associates with the T cell antigen receptor (TCR) and is found on all mature human T lymphocytes. CD3 can be used for the identification of normal and malignant T lymphocytes in paraffin embedded or frozen tissue sections. PC3/188A also detects CD3 in mammalian tissues. |
| References: | Jones et al. 1993. J Immunol 150(12): 5429-35. PMID: 8515069Read more |
| Notes: | Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
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