Anti CD68 Clone Y1/82A Profile

Antigen: CD68
Clone: Y1/82A
Subclass IgG2a
Species Raised In:

Mouse

Immunogen: Lysosomal contents of lung macrophages
Cross Reactivity:

Human

Applications: Frozen, Western
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Synonyms: Macrosialin
Relevance: CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. Y182A is more specific for monocytes/macrophages in blood and tissues compared with anti-CD68 clone KP1.
References:

Doussis et al. 1993. J Clin Pathol. 46:334-6. Read more
Micklem et al. 1989. Br J Haematol. 73:6-11. Read more
Pulford et al. 1990. Int Immunol. 2:973-80. Read more
Pulford et al. 1989. J Clin Pathol. 42:414-21. Read more

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.