Anti CD79a Clone JCB117 Profile

Antigen: CD79a
Clone: JCB117
Subclass IgG1
Species Raised In:

Mouse

Immunogen: Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence
Epitope:

Residues 202-216

Cross Reactivity:

Human

Applications: Frozen, Paraffin embedded, Western
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Synonyms: B-cell antigen receptor complex-associated protein alpha chain, IGA, MB1
Relevance: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.
References:

Korkolopoulou P et al. 1994. Histopathology 24(6):511-5. PMID: 7520411 Read more
Mason DY et al. 1995. Blood 86(4):1453-9. PMID: 7632952 Read more
Pilozzi E. Et al. 1998. J Pathol 186(2):140-3. PMID: 9924428 Read more

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.