Anti CD86 Clone BU63 Profile

Antigen: CD86
Clone: BU63
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: ARH 77 (B-lymphoblastoid cell line)
Cross Reactivity:

Human

Applications: FACS, Frozen, Immunoprecipitation, Western
Growth Conditions:

RPMI 1640 + 10% FCS

Fusion Partner: NS1
Relevance: CD86 is a 70 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily of cell surface receptors. CD86 is expressed on resting monocytes and dendritic cells, activated T and Natural Killer cells, and is rapidly induced on the surface of activated B-cells, suggesting that it is a major CD28 ligand and may play an important role for the co-stimulation of T-cells in the primary immune response. CD86 is also expressed by malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
References:

Caux C. et al. 1994. J.Exp.Med. 180(5):1841-7. PMID: 7525840 Read more
Engel et al. 1994. Blood 84(5):1402-7. PMID: 7520767 Read more
Giguere 2004. J.Virol. 78(12):6222-32. PMID: 15163715 Read more
Latour S. et al. 2001. J.Immunol. 167(5):2547-54. PMID: 11509594 Read more
Mauri D. et al. 1995. J.Immunol. 155(1):118-27. PMID: 7541409 Read more
Nozawa Y. et al. 1993. J.Pathol. 169(3):309-15. PMID: 8492223 Read more
O’Sullivan BJ and Thomas R. 2002. J.Immunol. 168(11):5491-8. PMID: 12023343 Read more
Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Notes:

Recommended for the inhibition of the mixed lymphocyte reaction (MLR) and the binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD 86 (B7-2).