Anti CD9 Clone P1/33/2 Profile

Antigen: CD9
Clone: P1/33/2
Subclass IgG1
Species Raised In:

Mouse

Immunogen: Acute lymphoblastic leukaemia cells
Cross Reactivity:

Human

Applications: FACS, Frozen
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and neurons and glial cells in the peripheral nervous system. CD9 mediates platelet aggregation and activation via FcgRIIa. It may play a role in cell migration.Antibodies against CD9 have utility in characterisation of acute leukaemias and in some cases AML and for functional studies on platelets
References:

Jennings LK, Crossno JT, White MM (1993) CD9 cluster workshop report: cell surface binding and functional analysis. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 2, Oxford University Press, Oxford, New York and Tokyo, p 1249-51

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.