Anti CDw75 Clone ZB55 Profile

Antigen: CDw75 (CD75s)
Clone: ZB55
Subclass IgM
Species Raised In:

Mouse

Immunogen: CD32 peptides
Cross Reactivity:

Human

Applications: FACS, Frozen, Paraffin embedded
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: CDw75 is a human leukocyte cell-surface antigen expressed in mature and activated B cells but not in B cells at earlier stages of development or in plasma cells. ZB55 can be used for studying germinal centre-derived B cell lymohomas.
References:

Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x
Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.