Anti CEACAM5 & 6 Clone By114 Profile

Antigen: Carcinoembryonic antigen-related cell adhesion molecules 5 & 6 (CEACAM5, CD66e; CEACAM6, CD66c) CD66 C and E subtypes (NCA)
Clone: By114
Subclass IgG1 kappa
Species Raised In:

Mouse

Immunogen: B cell Lymphoma cells
Cross Reactivity:

Human

Applications: FACS, Frozen, Immunoprecipitation, Paraffin embedded, Western
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: CEACAM is a member of the carcinoembryonic antigen family (a diagnostic marker for many cancers). Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).
References:

Mayne KM et al. 1993. Br J Haematol 83:30-38. PMID: 8435334 Read more
Tooze JA. 1995. Exp Hematol 23:1484-91. PMID: 8542935 Read more
Li C et al. 2006. Mol Cell Biol 17(9): 3848-50. PMID: 16807355 Read more
Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.