Anti EMA Clone E29 (LRF) Profile

Antigen: Epithelial Membrane Antigen (EMA)
Clone: E29 (LRF)
Subclass IgG2a
Species Raised In:

Mouse

Immunogen: Human milk fat globule membrane preparation
Cross Reactivity:

Human

Applications: Frozen, Paraffin embedded, Western
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: Marker for epithelial cells
References:

Cordell J et al. 1985. Br J Cancer 52(3):347-54. PMID: 3899155 Read more
Heyderman E et al. 1985. Br J Cancer 52(3):355-61. PMID: 3899156 Read more

Notes:

Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well.