Anti Factor VIII Clone RFF-VIII:c/8 Profile

Antigen: Factor VIII
Clone: RFF-VIII:c/8
Subclass IgG1 k
Species Raised In:

Mouse

Immunogen: Purified human factor VIII
Applications: Immunofluorescence, Neutralising, Western
Growth Conditions:

RPMI + 10% FCS; non-adherent; subculture every 2-3 days; split 1:5

Fusion Partner: P3/NS/1/Ag4
Relevance: The commonest severe congenital bleeding disorder in all races is haemophilia A. The characteristic defect is a lack of coagulation factor VIII:C. Factor VIII:C is a glycoprotein that functions as a cofactor for factor IXa which, in the presence of calcium and phospholipids, converts factor X to the activated form Xa. This antibody binds to human FVIII:C heavy chain and inhibits its function.
References:

Goodall AH et al. 1985. Thromb Haemost. 54(4):878-91. PMID: 3937279 Read more
Rotblat F et al. 1983. J Lab Clin Med. 101(5):736-46. PMID: 6403638 [RFF-VIII:c/8 was used to purify FVIII for this landmark study]Read more
Rotblat F et al. 1985. Biochemistry. 24(16):4294-300. PMID: 2413885Read more
Takase T et al. 1987. Br J Haematol. 66(4):497-502.PMID: 3117089Read more
Vehar GA et al. 1984. Nature. 1984 312(5992):337-42. PMID: 6438527Read more