| Antigen: | Gastrokine2 (GKN2, Human Homologue) |
| Clone: | GKN2 |
| Species Raised In: | Rabbit |
| Immunogen: | N-terminal residues 29-48 of the TFF2-AP binding protein |
| Positive Control: | Normal human (or mouse) stomach, with intact surface and/or pit epithelium. |
| Cross Reactivity: | Mouse, human |
| Applications: | Frozen, Immunofluorescence, Paraffin embedded, Western |
| Synonyms: | Blottin, Trefoil factor interactions(z) 1, TFIZ1, BLOT, GDDR |
| Relevance: | The murine protein Gastrokine 2 (GKN-2, previously known as blottin) binds to mouse Trefoil Factor Family (TFF) and is found in the gastric surface of foveolar epithelial cells. The protein has a human homologue (GDDR/TF1Z1) and has similarities with GKN-1 another gastric foveolar protein. GKN-2 is also seen in the reparative surface epithelium of the colon in the UACL of Crohn's disease. GKN-2 may inhibit cell proliferation in contrast to GKN-1 which is a mitogen. GKN-2 may play a role in gastro-intestinal mucosal maintenance and repair through interactions TFF. Previous work has shown the GKN-2 is down regulated in gastric cancer. |
| References: | Holness CL et al. 1995. J. Biol. Chem. 270(2): 877-84. PMID: 7822326 Read more |
| Notes: | Suggested Dilutions for western blot: 1:1000-1:2000 and ICH (paraffin embedded): 1:250 |
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