Anti HIF2A Clone EP190b Profile

Antigen: Hypoxia-inducible factor 2, alpha subunit (HIF2A)
Clone: EP190b
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: GST-human EPAS-1 (HIF2A) amino acids 535-631 fusion protein
Cross Reactivity:

Human

Applications: ELISA, Frozen, Paraffin embedded, Western
Growth Conditions:

RPMI + 10% FCS + HAT

Fusion Partner: NS1
Relevance: Induction of the Hif regulated genes, as a consequence of the microenvironment or genetic changes, is known to have an important role in the growth of experimental tumours. HIF2A is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF1A has been observed in varying subsets of tumour cells from various solid tumours. HIF2A is also a potent activator of the tie2 gene, which is known to be selectively expressed in endothelial cells.
References:

Beasley NJ et al. 2002. Cancer Res. 62(9):2493-7. PMID: 11980639 Read more
Giatromanolaki A et a. 2003. J. Pathol. 200(2):222-8. PMID: 12754744 Read more
Hui EP et al. 2002. Clin. Cancer. Res. 8(8):2595-604. PMID: 12171890 Read more
Talks KL et al. 2000. Am. J. Pathol. 157(2):411-21. PMID: 10934146 Read more
Wiesener MS et al. 1998. Blood. 92(7):2260-8. PMID: 9746763 Read more