Anti Integrin ß3 Clone F11 Profile

Antigen: Integrin ß3 (CD61)
Clone: F11
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.
Cross Reactivity:

Rat, Human (weak)

Applications: FACS, Frozen, Immunoprecipitation, Neutralising
Growth Conditions:

DMEM + 5% FCS

Fusion Partner: X63-Ag-8.653
Relevance: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets. The F11 epitope is different to the MHF4 epitope.
References:

Helfrich MH et al. 1992. J. Bone Miner. Res. 7(3):335-43. PMID: 1585836 Read more
Helfrich MH et al. 1992. J. Bone Miner. Res. 7(3):345-51. PMID: 1585837Read more
Thompson RD et al. 2000. J. Immunol. 165(1):426-34. PMID: 10861081 Read more
Shankar G et al.  1993. J Cell Sci. 105; 61-68. PMID: 7689577 Read more

Notes:

F11 may be used to identify ostoclasts in tissue sections and bone cell suspensions.