Anti Integrin ß3 Clone Y2/51 Profile

Antigen: Integrin ß3 (CD61; GPIIIa)
Clone: Y2/51
Subclass IgG1
Species Raised In:

Mouse

Immunogen: Phytohaemagglutin-stimulated peripheral blood cells
Cross Reactivity:

Human

Applications: FACS, Frozen, Paraffin embedded, Western
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: CD61 is expressed by megakaryocytes, platelets and osteoclasts. Y2/51 is widely used as a marker of megakaryocytes in bone marrow trephines.
References:

Gatter KC et al. 1988.Histopathology 13:257-67. PMID: 3192191Read more
Nachman RL & Leung LL 1982. J Clin Invest 69:263-9. PMID: 6460044 Read more
Vinci G et al. 1984. Br J Haematol 56:589-605. PMID: 6231944Read more

Notes:

Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well.