Anti Keratin 14 Clone LL001 Profile

Antigen: Keratin 14
Clone: LL001
Subclass IgG2a k
Species Raised In:

Balb/c

Immunogen: Peptide (last 16 carboxy-terminal amino acids of human keratin) coupled to thyroglobulin by the extra Cys added at the N terminus.
Cross Reactivity:

Human, Murine, Porcine

Applications: Frozen, Immunofluorescence, Paraffin embedded, Western
Growth Conditions:

DMEM + 5% FCS

Fusion Partner: Sp2/0Ag.14
Relevance: Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types.
References:

Leigh IM et al J Invest Dermatol. 1988 May;90(5):639-42. (Erratum in: J Invest Dermatol 1989 Jan;92(1):135.)  PMID: 3283248 Read more
Purkis P et al. 1990. J. Cell Sci. 97:39-50. PMID: 1701769 Read more
D’Alessandro M et al. 2002. J. Cell Sci. 115(22):4341-51. PMID: 12376565 Read more
Karashima T & Watt FM. 2002. J. Cell Sci. 115(24):5027-37.  PMID: 12432088 Read more

Notes:

LL001 may be used on formalin fixed sections but is more effective on unfixed frozen sections.