| Antigen: | Neutrophil elastase |
| Clone: | NP57 |
| Subclass | IgG1 kappa |
| Species Raised In: | Mouse |
| Immunogen: | Neutrophil granule proteins |
| Cross Reactivity: | Human |
| Applications: | Frozen, Immunoprecipitation, Paraffin embedded, Western |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | NS1 |
| Relevance: | Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leucocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis. NP57 is usefull for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection ofn eutrophils in paraffin sections |
| References: | Pulford K.A.F. et al. 1988. J Clin Pathol 41:853-60. PMID: 2844860Read more |
| Notes: | This cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
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