Anti PIP2 Clone PIP2 2C11 Profile

Antigen: Phosphatidylinositol biphosphate (PIP2)
Clone: PIP2 2C11
Subclass IgM
Species Raised In:

Balb/c

Immunogen: Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns
Positive Control:

To test the Ab efficiency use a lipid blot assay as described in References above.

Cross Reactivity:

PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3.

Applications: ELISA, Immunoprecipitation, Neutralising
Growth Conditions:

DMEM + 5% FCS

Fusion Partner: Sp2/0Ag.14
Relevance: PtdIns 4,5-P2 is a membrane phospholipid and an important component of signal transduction pathways.
References:

Thomas CL et al. 1999. Biochem. Soc. Trans. 27:648-52. PMID: 10917659Read more
Osborne SL et al. 2001. J. Cell Sci. 114:2501-11. PMID: 11559758Read more

Notes:

It is recomended to use a lipid blot assay or nuclear staining to test this antibody.