Anti SIGLEC-2 Clone 4KB128 Profile

Antigen: Sialic acid binding Ig-like lectin 2 (SIGLEC-2, CD22, BL-CAM)
Clone: 4KB128
Subclass IgG1 kappa
Species Raised In:

Mouse

Immunogen: B cell lymphoma cells
Cross Reactivity:

Human

Applications: FACS, Frozen
Growth Conditions:

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Fusion Partner: NS1
Relevance: SIGLEC-2 is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias.
References:

Campana D et al. 1985. J Immunol 134(3):1524-30. PMID: 3918103 Read more
Mason DY et al. 1987. Blood 69(3):836-40. PMID: 3101766 Read more
Law CL et al. 1994. Immunol Today 15:442-9. PMID: 7945784 Read more

Notes:

Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well.