| Antigen: | Sialic acid binding Ig-like lectin 2 (SIGLEC-2, CD22, BL-CAM) |
| Clone: | TPD144 |
| Subclass | IgG2a |
| Species Raised In: | Mouse |
| Immunogen: | B cell lymphoma cells |
| Epitope: | Surface |
| Cross Reactivity: | Human |
| Applications: | FACS, Frozen |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | NS1 |
| Relevance: | SIGLEC-2 (CD22) is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias. |
| Notes: | Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
© Cancer Research Technology 2012