| Antigen: | Spastin |
| Clone: | Sp 3G11/1 |
| Subclass | IgG2a |
| Species Raised In: | Balb/c |
| Immunogen: | Recombinant human spastin |
| Positive Control: | HeLa cell or rat brain extract |
| Cross Reactivity: | Human, mouse, rat |
| Applications: | ELISA, Immunofluorescence, Western |
| Growth Conditions: | DMEM + 5% FCS |
| Fusion Partner: | Sp2/0Ag.14 |
| Synonyms: | SPG 4, Spastic paraplegia 4 antibody |
| Relevance: | Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. |
| Notes: | Suggested dilution for western blot: 1:500 |
© Cancer Research Technology 2012