| Antigen: | Spectrin |
| Clone: | RG/26 |
| Subclass | IgG2a |
| Species Raised In: | Mouse |
| Immunogen: | Red cell membranes |
| Cross Reactivity: | Human |
| Applications: | Frozen, Western |
| Growth Conditions: | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Fusion Partner: | NS1 |
| Relevance: | Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death. |
| Notes: | Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well. |
© Cancer Research Technology 2012