Anti TAL-1 Clone 2TL75 Profile

Antigen: TAL-1
Clone: 2TL75
Subclass IgG1 Kappa
Species Raised In:

Mouse

Immunogen: Tal-1 Recombinant protein
Cross Reactivity:

Human

Applications: Immunohistochemistry, Immunoprecipitation
Growth Conditions:

It is advised that cells are grown in the following medium:
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.

Fusion Partner: P3/NS1/1-Ag4-1 (NS-1)
Synonyms: T-cell acute lymphocytic leukemia protein 1, T-cell leukemia/lymphoma protein 5, Class A basic helix-loop-helix protein 17, bHLHa17, BHLHA17
Relevance: TAL-1 is a member of the helix-loop-helix family of transcription factors. Overexpression of the TAL-1 gene is the most common molecular abnormality found in human T cell leukaemia.
References:

Bernard M, Smit L, Macintyre E, Matthieu-Mahul D, Pulford K (1995) Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain. Blood 85:3356-3357. PMID: 7756670
Pulford K, Lecointe N, Leroy-Viard K, Jones M, Mathieu-Mahul D, Mason DY (1995) Expression of TAL-1 proteins in human tissues. Blood 85:675-684. PMID: 7833471
Delabesse E, Bernard M, Meyer V, Smit L, Pulford K, Cayuela JM, Ritz J, Bourquelot P, Strominger JL, Valensi F, Macintyre EA (1998) TAL1 expression does not occur in the majority of T-ALL blasts. Br J Haematol 102:449-457. PMID: 9695959
Notes:

Please note that this cell line has not been weaned out of HAT.