| Antigen: | TIGAR |
| Clone: | 9C10 |
| Subclass | IgG2b |
| Species Raised In: | Mouse |
| Immunogen: | A 15-amino acid peptide corresponding to the exon COOH-terminal region of human TIGAR protein (CMNLQDHLNGLTETR). |
| Cross Reactivity: | Human |
| Applications: | Immunoprecipitation, Western |
| Growth Conditions: | DMEM + 10% Foetal Clone I (Hyclone cat no. SH30080.03) |
| Fusion Partner: | Sp2/0Ag.14 |
| Relevance: | TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis. |
| References: | Bensaad K, Tsuruta A et al. Cell. 2006 Jul 14;126(1):107-20. PMID: 16839880 Read more |
| Notes: | Suggested dilution of 1:5000 |
© Cancer Research Technology 2012