Anti Ufd1 Clone 5e2 Profile
| Antigen: | Ufd1 |
| Clone: | 5e2 |
| Subclass | IgG2a |
| Species Raised In: | Balb/c |
| Immunogen: | Full length mouse Ufd1 expressed in E. coli |
| Epitope: | Residues 115-220 |
| Positive Control: | HeLa or HEK293 cell lysate. |
| Cross Reactivity: | Human, Rat, Mouse, Xenopus laevis |
| Applications: | ELISA, Immunofluorescence, Immunoprecipitation, Western |
| Growth Conditions: | DMEM + 10% FCS |
| Fusion Partner: | Sp2/0Ag.14 |
| Relevance: | The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis. |
| References: | Hetzer et al., 2001. Nature Cell Biology. 3, 1086 -91. PMID: 11781570 Read more |
| Notes: | Recommended Concentrations: Western Blot: 0.5 ug/ml Immunofluorescence: 5 ug/ml, (formaldehyde-fixed) |
