Anti VWF Clone RFF-VIII:R/2 Profile

Antigen: von Willebrand Factor (VWF)
Clone: RFF-VIII:R/2
Subclass IgG1 k
Species Raised In:

Mouse

Immunogen: Purified human Factor VIII-VWF complex
Cross Reactivity:

Baboon.

Applications: ELISA, FACS, Frozen, Immunofluorescence, Immunoprecipitation, Neutralising, Paraffin embedded, Western
Growth Conditions:

RPMI + 10% FCS; non-adherent; subculture every 2-3 days; split 1:5

Fusion Partner: P3/NS/1/Ag4
Synonyms: F8VWF
Relevance: The monoclonal antibody RFF-VIII:R/1 specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/1 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg. This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits its binding to platelets. Very similar in characteristics to RFF-VIII:R/2
References:

Chand S et al. 1986. Thrombosis & Haemostasis. 55: 318-24. PMID: 2428124Read more
Goodall AH et al. 1985. British Journal of Haematology. 59: 565-77. PMID: 2580547Read more
McCraw A et al. 1987. Thrombosis Research. 45: 101-8. PMID: 3494325Read more
Murdock PJ et al. 1997. Thrombosis & Haemostasis. 78: 1272-7. PMID: 9364997Read more
Rotblat F et al. 1985. Biochemistry. 24: 4294-300. PMID: 2413885 [Use of RFF-VIII:R/1 in the purification of FVIII]Read more
Takase T et al. 1987. British Journal of Haematology. 66: 497-502. PMID: 3117089Read more