Anti Zebrafish Basolateral Pole of Cells Clone FIS 2H9/1 Profile

Antigen: Zebrafish Basolateral Pole of Cells
Clone: FIS 2H9/1
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: Lysate of zebrafish intestine
Cross Reactivity:

Zebrafish

Applications: Immunofluorescence, Western
Fusion Partner: Sp2/0Ag.14
Relevance: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 2H9 recognises a membrane-associated epitope on the gut epithelial cells, concentrated basolaterally. In one month old zebrafish it appears especially prominent in the basal parts of the elongated cells of the villi. This antibody is a useful marker of apicobasal polarity in the epithelium.
References:

Crosnier et al. Development. 2005. 132: 1093-104. PMID: 15689380Read more

Notes:

Recommended for use with formalin fixed gut sections of zebrafish. Suggested Dilution: 1:200