Anti Zebrafish DeltaD Clone zdd2 Profile

Antigen: Zebrafish DeltaD extracellular domain
Clone: zdd2
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: Extracellular domain; DeltaD peptide
Epitope:

Zebrafish DeltaD protein extracellular domain

Positive Control:

HEK293 cells transfected with deltaD; and zebrafish embryo

Cross Reactivity:

Zebrafish

Applications: ELISA, FACS, Frozen, Immunofluorescence, Western
Fusion Partner: Sp2/0Ag.14
Relevance: Delta proteins activate Notch through a binding reaction that depends on their extracellular domains; they play a key role in the control of cell differentiation by lateral inhibition. DeltaD is widely expressed in subsets of cells in the nervous system, mesoderm, gut, and elsewhere. Deltas associate with membrane-associated scaffolding proteins MAGI1, MAGI2 and MAGI3. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.
References:

Itoh M et al. Dev Cell. 2003. 4: 67-82. PMID: 12530964Read more
Wright GJ et al. Development. 2004. 131: 5659-6. PMID: 15509766 Read more
Crosnier et al. Development. 2005. 132: 1093-104. PMID: 15689380Read more

Notes:

Recommended for use in formalin fixed tissue sections of zebrafish.