Anti Zebrafish gut absorptive cell epitopes Clone FIS 4E8/1 Profile

Antigen: Zebrafish gut absorptive cell epitopes
Clone: FIS 4E8/1
Subclass IgG1
Species Raised In:

Balb/c

Immunogen: Lysate of zebrafish intestines
Cross Reactivity:

Zebrafish

Applications: Immunoprecipitation, Western
Fusion Partner: Sp2/0Ag.14
Relevance: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli). This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
References:

Crosnier et al. Development. 2005. 132: 1093-104. PMID: 15689380 Read more
Dong et al. Nature Genetics. 2007. 39: 397-402. PMID: 17259985 Read more
Bates et al. Developmental Biology. 2006. 297: 374-86. PMID: 16781702 Read more
Matthews et al. Developmental Dynamics. 2008. 237: 124-31. PMID: 18095340 Read more

Notes:

Suggested Dilution: 1:200. 4E8/1 may be used on formalin fixed gut sections of zebrafish.